Protein Sequencing Service by Edman Degradation

Protein sequencing service by Edman degradation is a method used to identify the N-terminal sequence of proteins. Edman degradation is one of the most mature methods for N-terminal sequence analysis of proteins and has a wide range of applications. Compared with MS, Edman sequencing provides more accurate protein sequence data.

Service Advantages

1. High Sensitivity Detection

MtoZ Biolabs uses the SHIMADZU PPSQ-33A analyzer, which integrates an advanced HPLC system and UV-VIS SPD-20A detector. This setup is capable of detecting PTH-AA at low picomole level, ensuring high precision and sensitivity in detection. 

2. Broad Applicability 

Protein sequencing service by Edman degradation can analyze up to 30 amino acid residues and is particularly effective for confirming the N-terminal sequences of antibodies, vaccines, peptide therapeutics, protein drugs, recombinant protein, ect. 

3. High-Resolution Separation

The advanced HPLC system can accurately distinguish amino acids, i.e. I/L, and Q/K, allowing efficient analysis of complex samples, including proteins with post-translational modifications or specific domains. 

Sample Submission Requirements

1. Electroblotted Samples

Proteins separated by SDS-PAGE should be transferred to PVDF membranes. It is recommended to stain the membranes with Coomassie Brilliant Blue or Ponceau S, avoiding silver staining. Thorough washing of the PVDF membranes is required to remove potential contamination from glycine buffer. 

2. Liquid Samples

Samples should be prepared at a concentration of 1-10 µg with a purity greater than 90%. Buffers should exclude Tris, glycine, SDS, and other potential contaminants to ensure accurate sequencing. 

MtoZ Biolabs, an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. 

Interested in learning more about how our protein sequencing service by Edman degradation can enhance your research? Contact us today for a free consultation and discover how we can help accelerate your research.

Contact Detail:

Company Name: MtoZ Biolabs
Contact Person: Terry
Email: Send Email
Phone No: +1-857-362-9535
Address: 155 Federal Street, Suite 700, Boston, MA 02110, USA
City: Boston
State: MA
Country: United States
Website Url: https://www.mtoz-biolabs.com/